Extract-N-Amp: Sample to PCR in under 15 minutes.

T9424

Sigma

 

TRI Reagent®

For processing tissues, cells cultured in monolayer or cell pellets

Synonym:TRI Reagent® RNA Isolation Reagent
MDL number:MFCD00213058

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Description

Frequently Asked QuestionsLive Chat and Frequently Asked Questions are available for this Product.
ApplicationTRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski.1 The RNA isolation method based on this reagent is widely used and proven for RNA applications2. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.
Features and Benefits• Quick and convenient reagent for use in the simultaneous isolation of RNA, DNA and protein.
• Performs well with large or small amounts of tissue or cells and many samples can be simultaneously extracted.
• One of the most effective methods for isolating total RNA. Purifications can be completed in only one hour starting with fresh tissue or cells.
Legal InformationTRI Reagent is a registered trademark of Molecular Research Center, Inc.

Properties

usage mL sufficient for 107 cells
  mL sufficient for 100 mg tissue
storage temp.2-8°C

Safety

Hazard CodesT
Risk Statements23/24/25-32-34-48/20/21/22-52/53-68
Safety Statements26-36/37/39-45-61
RIDADRUN 2821 6.1/PG 3
WGK Germany2
RTECSUN1760000
Flash Point(F)174 °F
Flash Point(C)79 °C

References

Cited Reference1. Chomcznski, P., and Mackey, K., Modification of the Tri Reagent procedure for isolation of RNA from polysaccharide- and proteoglycan-rich sources. Biotechniques 19, 924, (1995)
 2. Chomczynski, P., and Sacchi, N., Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156, (1987)
referenceChomczynski, P., A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques 15, 532-34,536-37, (1993)